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Computational Design of a DNA- and Fc-Binding Fusion Protein
Joint Authors
Dybowski, J. Nikolaj
Heider, Dominik
Armano, Giuliano
Kuhn, Oliver
Winkler, Jonas
Ledda, Filippo
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Computational design of novel proteins with well-defined functions is an ongoing topic in computational biology.
In this work, we generated and optimized a new synthetic fusion protein using an evolutionary approach.
The optimization was guided by directed evolution based on hydrophobicity scores, molecular weight, and secondary structure predictions.
Several methods were used to refine the models built from the resulting sequences.
We have successfully combined two unrelated naturally occurring binding sites, the immunoglobin Fc-binding site of the Z domain and the DNA-binding motif of MyoD bHLH, into a novel stable protein.
American Psychological Association (APA)
Winkler, Jonas& Armano, Giuliano& Dybowski, J. Nikolaj& Kuhn, Oliver& Ledda, Filippo& Heider, Dominik. 2011. Computational Design of a DNA- and Fc-Binding Fusion Protein. Advances in Bioinformatics،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-473057
Modern Language Association (MLA)
Winkler, Jonas…[et al.]. Computational Design of a DNA- and Fc-Binding Fusion Protein. Advances in Bioinformatics No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-473057
American Medical Association (AMA)
Winkler, Jonas& Armano, Giuliano& Dybowski, J. Nikolaj& Kuhn, Oliver& Ledda, Filippo& Heider, Dominik. Computational Design of a DNA- and Fc-Binding Fusion Protein. Advances in Bioinformatics. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-473057
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473057