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Encapsulation of Protein-Polysaccharide HIP Complex in Polymeric Nanoparticles
Joint Authors
Khurana, Varun
Parenky, Ashwin
Mitra, Ashim K.
Gaudana, Ripal
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The objective of the present study is to formulate and characterize a nanoparticulate-based formulation of a macromolecule in a hydrophobic ion pairing (HIP) complex form.
So far, HIP complexation approach has been studied only for proteins with molecular weight of 10–20 kDa.
Hence, we have selected bovine serum albumin (BSA) having higher molecular weight (66.3 kDa) as a model protein and dextran sulphate (DS) as a complexing polymer to generate HIP complex.
We have prepared and optimized the HIP complex formation process of BSA with DS.
Ionic interactions between basic amino acids of BSA with sulphate groups of DS were confirmed by FTIR analysis.
Further, nanoparticles were prepared and characterized with respect to size and surface morphology.
We observed significant entrapment of BSA in nanoparticles prepared with minimal amounts of PLGA polymer.
Finally, results of circular dichroism and intrinsic fluorescence assay have clearly indicated that HIP complexation and method of nanoparticle preparation did not alter the secondary and tertiary structures of BSA.
American Psychological Association (APA)
Gaudana, Ripal& Khurana, Varun& Parenky, Ashwin& Mitra, Ashim K.. 2011. Encapsulation of Protein-Polysaccharide HIP Complex in Polymeric Nanoparticles. Journal of Drug Delivery،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-473102
Modern Language Association (MLA)
Gaudana, Ripal…[et al.]. Encapsulation of Protein-Polysaccharide HIP Complex in Polymeric Nanoparticles. Journal of Drug Delivery No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-473102
American Medical Association (AMA)
Gaudana, Ripal& Khurana, Varun& Parenky, Ashwin& Mitra, Ashim K.. Encapsulation of Protein-Polysaccharide HIP Complex in Polymeric Nanoparticles. Journal of Drug Delivery. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-473102
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473102