Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis
Joint Authors
Heinemann, Marcos Bryan
Reis, Jenner Karlisson Pimenta dos
Leite, Rômulo Cerqueira
Sales, Érica Bravo
Fonseca, Antônio Augusto
Magalhães, Cristina Gonçalves
D'Ambros, Régia Maria
Ciacci Zanella, Janice R.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-24
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky's disease.
The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV.
The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis.
The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI.
Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes.
The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs.
The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1.
American Psychological Association (APA)
Fonseca, Antônio Augusto& Magalhães, Cristina Gonçalves& Sales, Érica Bravo& D'Ambros, Régia Maria& Ciacci Zanella, Janice R.& Heinemann, Marcos Bryan…[et al.]. 2011. Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis. ISRN Microbiology،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-473116
Modern Language Association (MLA)
Fonseca, Antônio Augusto…[et al.]. Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis. ISRN Microbiology No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-473116
American Medical Association (AMA)
Fonseca, Antônio Augusto& Magalhães, Cristina Gonçalves& Sales, Érica Bravo& D'Ambros, Régia Maria& Ciacci Zanella, Janice R.& Heinemann, Marcos Bryan…[et al.]. Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis. ISRN Microbiology. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-473116
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473116
