Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction
Joint Authors
Welinder, Charlotte
Hansson, Stefan R.
Centlow, Magnus
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-09-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The human placenta is a difficult tissue to work with using proteomic technology since it contains large amounts of lipids and glycogen.
Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing.
In order to gain the best possible protein separation on 2D-PAGE, an optimized sample preparation protocol for placental proteins was developed.
Two different buffers, urea/CHAPS and Hepes, were used for solubilization in combination with six different precipitation methods.
The removal of glycogen from the samples by centrifugation was crucial for the final proteome maps.
Solubilization with urea/CHAPS in combination with dichloromethane/methanol or acidified acetone proved to be the best precipitation procedures.
When applied to clinical placenta samples apolipoprotein A1 was found to be accumulated in the preeclamptic placenta, where it may either have a nutritional effect or act as a modifier of signal transduction.
American Psychological Association (APA)
Centlow, Magnus& Hansson, Stefan R.& Welinder, Charlotte. 2009. Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-473157
Modern Language Association (MLA)
Centlow, Magnus…[et al.]. Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-473157
American Medical Association (AMA)
Centlow, Magnus& Hansson, Stefan R.& Welinder, Charlotte. Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction. Journal of Biomedicine and Biotechnology. 2009. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-473157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473157