Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients
Joint Authors
Cohen-Mazor, Meital
Sela, Shifra
Kristal, Batya
Mazor, Rafi
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aims.
To test the hypothesis that primed PMNLs in blood of chronic kidney disease patients release the active form of elastase and cathepsin G causing degradation of vital proteins and promote tissue damage.
Methods.
RT-PCR, immunocytochemical staining, immunoblotting, and FACS analyses were used to study these enzymes in hemodialysis patients (HD) versus healthy normal controls (NC).
Using PMNLs and endothelial cells cocultivation system we measure the effect of HD PMNLs on the endothelial VE-cadherin, an essential protein for maintaining endothelial integrity.
Results.
Levels of elastase and cathepsin G were reduced in PMNLs of HD patients, while mRNA enzymes levels were not different.
Elevated levels of the active form of these enzymes were found in blood of HD patients compared to NC.HD plasma had higher levels of soluble VE-cadherin present in three molecular forms: whole 140 kDa molecule and two fragments of 100 and 40 kDa.
Cocultivation studies showed that primed PMNLs cleave the endothelial cadherin, resulting in a 100 kDa fragment.
Conclusions.
Elastase and cathepsin G are elevated in the plasma of HD patients, originating from primed PMNLs.
In these patients, chronic elevation of these enzymes contributes to cleavage of VE-cadherin and possible disruption of endothelial integrity.
American Psychological Association (APA)
Cohen-Mazor, Meital& Mazor, Rafi& Kristal, Batya& Sela, Shifra. 2014. Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-473245
Modern Language Association (MLA)
Cohen-Mazor, Meital…[et al.]. Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-473245
American Medical Association (AMA)
Cohen-Mazor, Meital& Mazor, Rafi& Kristal, Batya& Sela, Shifra. Elastase and Cathepsin G from Primed Leukocytes Cleave Vascular Endothelial Cadherin in Hemodialysis Patients. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-473245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473245