Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors
Joint Authors
Sharma, Ashwani Kumar
Mahato, Manohar
Yadav, Santosh
Kumar, Pradeep
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-24
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Previously, we demonstrated that 6-(N,N,N′,N′-tetramethylguanidinium chloride)-hexanoyl-polyethylenimine (THP) polymers exhibited significantly enhanced transfection efficiency and cell viability.
Here, in the present study, we have synthesized a series of N,N,N′,N′-tetramethylguanidinium-polyethylenimine (TP1-TP5) polymers via a single-step reaction involving peripheral primary amines of bPEI and varying amounts of 2-(1H-benzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU).
These polymers were found to interact efficiently with negatively charged pDNA and formed stable complexes in the size range of ~240–450 nm.
Acid-base titration profiles revealed improved buffering capacity of TP polymers as compared to bPEI.
Transfection and cytotoxicity assays performed with TP/pDNA complexes on HEK293, CHO, and HeLa cells showed significantly higher transfection efficiency and cell viability with one of the complexes, TP2/pDNA complex, exhibited the highest transfection efficiency (~1.4–2.3-fold) outcompeting native bPEI and the commercially available transfection reagent, Lipofectamine 2000.
Compared to previously reported THP polymers, the transfection efficiency of TP/pDNA complexes was found to be lower, as examined by flow cytometry.
These results highlight the importance of the hydrophobic C-6 linker in THP polymers in forming compact nanostructures with pDNA, which might lead to efficient uptake and internalization of the complexes; however, the projected TP polymers offer an advantage of their rapid and economical one-step synthesis.
American Psychological Association (APA)
Mahato, Manohar& Yadav, Santosh& Kumar, Pradeep& Sharma, Ashwani Kumar. 2014. Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-473251
Modern Language Association (MLA)
Mahato, Manohar…[et al.]. Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-473251
American Medical Association (AMA)
Mahato, Manohar& Yadav, Santosh& Kumar, Pradeep& Sharma, Ashwani Kumar. Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-473251
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473251