Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection : Application to a Clinical Study
Joint Authors
Novotná, Pavla
Kand’ár, Roman
Drábková, Petra
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A method is described here for the simultaneous determination of retinol, α-tocopherol, lycopene, and β-carotene in human plasma.
The effectiveness of various protein precipitants and extraction solvents was tested.
After adequate sample preparation, the samples were injected directly into the HPLC system.
The separation was realized on an analytical reversed-phase column with a UV-Vis detection.
The analytical performance of this method was satisfactory.
The intraassay and interassay coefficients of variation were below 10%.
The recoveries were as follows: 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for α-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for β-carotene.
The levels of selected fat-soluble vitamins in plasma of patients with cardiovascular disease were measured and discussed.
American Psychological Association (APA)
Kand’ár, Roman& Novotná, Pavla& Drábková, Petra. 2012. Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection : Application to a Clinical Study. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-473281
Modern Language Association (MLA)
Kand’ár, Roman…[et al.]. Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection : Application to a Clinical Study. Journal of Chemistry No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-473281
American Medical Association (AMA)
Kand’ár, Roman& Novotná, Pavla& Drábková, Petra. Determination of Retinol, α-Tocopherol, Lycopene, and β-Carotene in Human Plasma Using HPLC with UV-Vis Detection : Application to a Clinical Study. Journal of Chemistry. 2012. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-473281
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473281