Difference in Early Activation of NF-κB and MCP-1 in Acinar-Cell-Rich versus Fibrotic Human Pancreas Exposed to Surgical Trauma and Hypoxia
Joint Authors
Sand, Juhani
Laaninen, Matias
Nordback, Isto
Bläuer, Merja
Laukkarinen, Johanna
Source
Gastroenterology Research and Practice
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
Previously we have shown that a pancreas with over 40% acinar cells is exposed to postoperative pancreatitis and other complications after pancreaticoduodenectomy (PD).
Our aim was to analyze the expression of NF-κB and MCP-1 in the cut edge of human pancreas after PD in both acinar-cell-rich and fibrotic pancreata.
Methods.
Several pancreatic samples from six patients, three with acinar-cell-rich and three with fibrotic pancreata, were exposed to surgical trauma in PD, and thereafter to hypoxemia for 15 minutes, 2–2.5 hours, 4 hours, or 6 hours, to mimic postoperative conditions of the pancreatic remnant in a patient.
Immunohistochemical analysis of inflammation markers (NF-κB, MCP-1) was performed.
Results.
In the acinar-cell-rich pancreata, intra-acinar NF-κB and MCP-1 expression increased from mild at 15 minutes to high during the first 4 hours, whereas in ductal cells MCP-1 staining was highly intense at both time points.
Acinar cell NF-κB and MCP-1 expression and ductal cell MCP-1 expression were also observed in the fibrotic pancreata, but the activation remained low throughout the 6 hours.
Conclusions.
In acinar-cell-rich pancreas, an extensive inflammatory cascade begins almost immediately after surgical trauma.
Fibrosis may limit the progression of inflammatory process in pancreas.
American Psychological Association (APA)
Laaninen, Matias& Bläuer, Merja& Sand, Juhani& Nordback, Isto& Laukkarinen, Johanna. 2014. Difference in Early Activation of NF-κB and MCP-1 in Acinar-Cell-Rich versus Fibrotic Human Pancreas Exposed to Surgical Trauma and Hypoxia. Gastroenterology Research and Practice،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-473294
Modern Language Association (MLA)
Laaninen, Matias…[et al.]. Difference in Early Activation of NF-κB and MCP-1 in Acinar-Cell-Rich versus Fibrotic Human Pancreas Exposed to Surgical Trauma and Hypoxia. Gastroenterology Research and Practice No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-473294
American Medical Association (AMA)
Laaninen, Matias& Bläuer, Merja& Sand, Juhani& Nordback, Isto& Laukkarinen, Johanna. Difference in Early Activation of NF-κB and MCP-1 in Acinar-Cell-Rich versus Fibrotic Human Pancreas Exposed to Surgical Trauma and Hypoxia. Gastroenterology Research and Practice. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-473294
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473294