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Stable Suppression of Lactate Dehydrogenase Activity during Anoxia in the Foot Muscle of Littorina littorea and the Potential Role of Acetylation as a Novel Posttranslational Regulatory Mechanism
Joint Authors
Dawson, Neal J.
Bell, Ryan A. V.
Shahriari, Ali
Storey, Kenneth B.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-10-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The intertidal marine snail, Littorina littorea, has evolved to withstand extended bouts of oxygen deprivation brought about by changing tides or other potentially harmful environmental conditions.
Survival is dependent on a strong suppression of its metabolic rate and a drastic reorganization of its cellular biochemistry in order to maintain energy balance under fixed fuel reserves.
Lactate dehydrogenase (LDH) is a crucial enzyme of anaerobic metabolism as it is typically responsible for the regeneration of NAD+, which allows for the continued functioning of glycolysis in the absence of oxygen.
This study compared the kinetic and structural characteristics of the D-lactate specific LDH (E.C.
1.1.1.28) from foot muscle of aerobic control versus 24 h anoxia-exposed L.
littorea.
Anoxic LDH displayed a near 50% decrease in Vmax (pyruvate-reducing direction) as compared to control LDH.
These kinetic differences suggest that there may be a stable modification and regulation of LDH during anoxia, and indeed, subsequent dot-blot analyses identified anoxic LDH as being significantly less acetylated than the corresponding control enzyme.
Therefore, acetylation may be the regulatory mechanism that is responsible for the suppression of LDH activity during anoxia, which could allow for the production of alternative glycolytic end products that in turn would increase the ATP yield under fixed fuel reserves.
American Psychological Association (APA)
Shahriari, Ali& Dawson, Neal J.& Bell, Ryan A. V.& Storey, Kenneth B.. 2013. Stable Suppression of Lactate Dehydrogenase Activity during Anoxia in the Foot Muscle of Littorina littorea and the Potential Role of Acetylation as a Novel Posttranslational Regulatory Mechanism. Enzyme Research،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-473384
Modern Language Association (MLA)
Shahriari, Ali…[et al.]. Stable Suppression of Lactate Dehydrogenase Activity during Anoxia in the Foot Muscle of Littorina littorea and the Potential Role of Acetylation as a Novel Posttranslational Regulatory Mechanism. Enzyme Research No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-473384
American Medical Association (AMA)
Shahriari, Ali& Dawson, Neal J.& Bell, Ryan A. V.& Storey, Kenneth B.. Stable Suppression of Lactate Dehydrogenase Activity during Anoxia in the Foot Muscle of Littorina littorea and the Potential Role of Acetylation as a Novel Posttranslational Regulatory Mechanism. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-473384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473384