Bacteriostatic Antimicrobial Combination : Antagonistic Interaction between Epsilon-Viniferin and Vancomycin against Methicillin-Resistant Staphylococcus aureus
Joint Authors
Basri, Dayang Fredalina
Abdul Shukor, Nur Indah
Xian, Lee Wee
Latip, Jalifah
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Stilbenoids have been considered as an alternative phytotherapeutic treatment against methicillin-resistant Staphylococcus aureus (MRSA) infection.
The combined effect of ε-viniferin and johorenol A with the standard antibiotics, vancomycin and linezolid, was assessed against MRSA ATCC 33591 and HUKM clinical isolate.
The minimum inhibitory concentration (MIC) value of the individual tested compounds and the fractional inhibitory concentration index (FICI) value of the combined agents were, respectively, determined using microbroth dilution test and microdilution checkerboard (MDC) method.
Only synergistic outcome from checkerboard test will be substantiated for its rate of bacterial killing using time-kill assay.
The MIC value of ε-viniferin against ATCC 33591 and johorenol A against both strains was 0.05 mg/mL whereas HUKM strain was susceptible to 0.1 mg/mL of ε-viniferin.
MDC study showed that only combination between ε-viniferin and vancomycin was synergistic against ATCC 33591 (FICI 0.25) and HUKM (FICI 0.19).
All the other combinations (ε-viniferin-linezolid, johorenol A-vancomycin, and johorenol A-linezolid) were either indifferent or additive against both strains.
However, despite the FICI value showing synergistic effect for ε-viniferin-vancomycin, TKA analysis displayed antagonistic interaction with bacteriostatic action against both strains.
As conclusion, ε-viniferin can be considered as a bacteriostatic stilbenoid as it antagonized the bactericidal activity of vancomycin.
These findings therefore disputed previous report that ε-viniferin acted in synergism with vancomycin but revealed that it targets similar site in close proximity to vancomycin’s action, possibly at the bacterial membrane protein.
Hence, this combination has a huge potential to be further studied and developed as an alternative treatment in combating MRSA in future.
American Psychological Association (APA)
Basri, Dayang Fredalina& Xian, Lee Wee& Abdul Shukor, Nur Indah& Latip, Jalifah. 2014. Bacteriostatic Antimicrobial Combination : Antagonistic Interaction between Epsilon-Viniferin and Vancomycin against Methicillin-Resistant Staphylococcus aureus. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-473425
Modern Language Association (MLA)
Basri, Dayang Fredalina…[et al.]. Bacteriostatic Antimicrobial Combination : Antagonistic Interaction between Epsilon-Viniferin and Vancomycin against Methicillin-Resistant Staphylococcus aureus. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-473425
American Medical Association (AMA)
Basri, Dayang Fredalina& Xian, Lee Wee& Abdul Shukor, Nur Indah& Latip, Jalifah. Bacteriostatic Antimicrobial Combination : Antagonistic Interaction between Epsilon-Viniferin and Vancomycin against Methicillin-Resistant Staphylococcus aureus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-473425
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473425