Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes
Joint Authors
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Actinomycetes are an important source of biologically active compounds, like antibiotics, antitumor agents, and immunosuppressors.
Genome sequencing is revealing that this class of microorganisms has larger genomes relative to other bacteria and uses a considerable fraction of its coding capacity (5–10%) for the production of mostly cryptic secondary metabolites.
To access actinomycetes biosynthetic capabilities or to improve the pharmacokinetic properties and production yields of these chemically complex compounds, genetic manipulation of the producer strains can be performed.
Heterologous expression in amenable hosts can be useful to exploit and to explore the genetic potential of actinomycetes and not cultivable but interesting bacteria.
Artificial chromosomes that can be stably integrated into the Streptomyces genome were constructed and demonstrated to be effective for transferring entire biosynthetic gene clusters from intractable actinomycetes into more suitable hosts.
In this paper, the construction of several shuttle Escherichia coli-Streptomyces artificial chromosomes is discussed together with old and new strategies applied to improve heterologous production of secondary metabolites.
American Psychological Association (APA)
Alduina, Rosa& Gallo, Giuseppe. 2012. Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-473458
Modern Language Association (MLA)
Alduina, Rosa& Gallo, Giuseppe. Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-473458
American Medical Association (AMA)
Alduina, Rosa& Gallo, Giuseppe. Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-473458
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473458