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Protein Interactions Investigated by the Raman Spectroscopy for Biosensor Applications
Joint Authors
Lagarde, F.
Durand-Thouand, M. J.
Daniel, Ph.
Jeyachandran, Y. L.
Thouand, G.
Pilard, J. F.
Kengne-Momo, R. P.
Source
International Journal of Spectroscopy
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Interaction and surface binding characteristics of staphylococcal protein A (SpA) and an anti-Escherichia coli immunoglobulin G (IgG) were studied using the Raman spectroscopy.
The tyrosine amino acid residues present in the α-helix structure of SpA were found to be involved in interaction with IgG.
In bulk interaction condition the native structure of proteins was almost preserved where interaction-related changes were observed in the overall secondary structure (α-helix) of SpA.
In the adsorbed state, the protein structure was largely modified, which allowed the identification of tyrosine amino acids involved in SpA and IgG interaction.
This study constitutes a direct Raman spectroscopic investigation of SpA and IgG (receptor-antibody) interaction mechanism in the goal of a future biosensor application for detection of pathogenic microorganisms.
American Psychological Association (APA)
Kengne-Momo, R. P.& Daniel, Ph.& Lagarde, F.& Jeyachandran, Y. L.& Pilard, J. F.& Durand-Thouand, M. J.…[et al.]. 2012. Protein Interactions Investigated by the Raman Spectroscopy for Biosensor Applications. International Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-473516
Modern Language Association (MLA)
Kengne-Momo, R. P.…[et al.]. Protein Interactions Investigated by the Raman Spectroscopy for Biosensor Applications. International Journal of Spectroscopy No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-473516
American Medical Association (AMA)
Kengne-Momo, R. P.& Daniel, Ph.& Lagarde, F.& Jeyachandran, Y. L.& Pilard, J. F.& Durand-Thouand, M. J.…[et al.]. Protein Interactions Investigated by the Raman Spectroscopy for Biosensor Applications. International Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-473516
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473516