HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form
Joint Authors
Uchadadiya, Nirav B.
Mehta, Falgun A.
Sanchaniya, Pinak M.
Source
Chromatography Research International
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A simple, precise, accurate, and reliable HPTLC method has been developed and validated for the analysis of EPE-Eperisone hydrochloride and PCM-Paracetamol in their combined dosage form.
Identification and analysis were performed on 100 mm × 100 mm layer thickness 0.2 mm, precoated silica gel G60-F254 aluminum sheet, prewashed with methanol, and dried in an oven at 50°C for 5 min.
Toluene : methanol : ethyl acetate : glacial acetic acid (4 : 3.5 : 2.5 : 0.05) (v/v/v/v) was used as mobile phase.
Calibration plots were established showing the dependence of response (peak area) on the amount chromatographed.
The validated calibration ranges were 200–700 ng/spot and 1300–4550 ng/spot for EPE and PCM with correlation coefficient (R2) 0.994 and 0.996, respectively.
Average % recovery was between 98.61–100.94% and 99.18–100.57% for EPE and PCM, respectively.
The spots were scanned at 248 nm in a reflectance mode.
The proposed method was validated as per ICH guidelines and successfully applied to the estimation of EPE and PCM in their combined tablet dosage form.
American Psychological Association (APA)
Uchadadiya, Nirav B.& Mehta, Falgun A.& Sanchaniya, Pinak M.. 2013. HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form. Chromatography Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-473678
Modern Language Association (MLA)
Uchadadiya, Nirav B.…[et al.]. HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form. Chromatography Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-473678
American Medical Association (AMA)
Uchadadiya, Nirav B.& Mehta, Falgun A.& Sanchaniya, Pinak M.. HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-473678
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473678