HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form

Joint Authors

Uchadadiya, Nirav B.
Mehta, Falgun A.
Sanchaniya, Pinak M.

Source

Chromatography Research International

Issue

Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2013-05-19

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Chemistry

Abstract EN

A simple, precise, accurate, and reliable HPTLC method has been developed and validated for the analysis of EPE-Eperisone hydrochloride and PCM-Paracetamol in their combined dosage form.

Identification and analysis were performed on 100 mm × 100 mm layer thickness 0.2 mm, precoated silica gel G60-F254 aluminum sheet, prewashed with methanol, and dried in an oven at 50°C for 5 min.

Toluene : methanol : ethyl acetate : glacial acetic acid (4 : 3.5 : 2.5 : 0.05) (v/v/v/v) was used as mobile phase.

Calibration plots were established showing the dependence of response (peak area) on the amount chromatographed.

The validated calibration ranges were 200–700 ng/spot and 1300–4550 ng/spot for EPE and PCM with correlation coefficient (R2) 0.994 and 0.996, respectively.

Average % recovery was between 98.61–100.94% and 99.18–100.57% for EPE and PCM, respectively.

The spots were scanned at 248 nm in a reflectance mode.

The proposed method was validated as per ICH guidelines and successfully applied to the estimation of EPE and PCM in their combined tablet dosage form.

American Psychological Association (APA)

Uchadadiya, Nirav B.& Mehta, Falgun A.& Sanchaniya, Pinak M.. 2013. HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form. Chromatography Research International،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-473678

Modern Language Association (MLA)

Uchadadiya, Nirav B.…[et al.]. HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form. Chromatography Research International No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-473678

American Medical Association (AMA)

Uchadadiya, Nirav B.& Mehta, Falgun A.& Sanchaniya, Pinak M.. HPTLC-Densitometric Analysis of Eperisone Hydrochloride and Paracetamol in Their Combined Tablet Dosage Form. Chromatography Research International. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-473678

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-473678