111Indium Labelling of Recombinant Activated Coagulation Factor VII : In Vitro and Preliminary In Vivo Studies in Healthy Rats
Joint Authors
Kjaer, Andreas
Bodholdt, Rasmus Poul
Hesse, Birger
Buch, Inge
Nalla, Amarnadh
Sigvardt, Maibritt
Source
International Journal of Molecular Imaging
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of this study is to investigate whether 111Indium-labelled recombinant FVIIa (rFVIIa) could be a potential radiopharmaceutical for localization of bleeding sources.
DTPA-conjugated rFVIIa was radiolabelled with 111In chloride.
In vitro binding efficiency of 111In-DTPA-rFVIIa to F1A2-Mab-sepharose was 99% in buffer, while it was 88–82% in serum.
The binding efficiency of 111In-DTPA-rFVIIa to TF (1–209)-sepharose was 48% in buffer whereas 39%–36% in serum, respectively.
In vivo experiment was conducted in healthy rats, and gamma camera images were taken immediately after iv.
administration of 1.6–1.8 MBq 111In-DTPA-rFVIIa up to 120–130 min.
Five min after administration of 111In-DTPA-rFVIIa, percentage of 111In activity was 6.0% in the cardiac region and 24.5% in the liver region.
After 2 hours activity was decreased to 3.3% in heart while it had increased to 42.0% in the liver.
The 111In-DTPA-rFVIIa might be a potential radiopharmaceutical for visualisation of tissues with significant TF expression such as acute bleeding lesions in the gastrointestinal tract.
American Psychological Association (APA)
Nalla, Amarnadh& Buch, Inge& Sigvardt, Maibritt& Bodholdt, Rasmus Poul& Kjaer, Andreas& Hesse, Birger. 2012. 111Indium Labelling of Recombinant Activated Coagulation Factor VII : In Vitro and Preliminary In Vivo Studies in Healthy Rats. International Journal of Molecular Imaging،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-473680
Modern Language Association (MLA)
Nalla, Amarnadh…[et al.]. 111Indium Labelling of Recombinant Activated Coagulation Factor VII : In Vitro and Preliminary In Vivo Studies in Healthy Rats. International Journal of Molecular Imaging No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-473680
American Medical Association (AMA)
Nalla, Amarnadh& Buch, Inge& Sigvardt, Maibritt& Bodholdt, Rasmus Poul& Kjaer, Andreas& Hesse, Birger. 111Indium Labelling of Recombinant Activated Coagulation Factor VII : In Vitro and Preliminary In Vivo Studies in Healthy Rats. International Journal of Molecular Imaging. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-473680
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473680