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Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line
Joint Authors
Abd Malek, Sri Nurestri
Karsani, Saiful Anuar
Lay, Ma Ma
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-22
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
2,4′,6-Trihydroxy-4-methoxybenzophenone was isolated from the ethyl acetate fraction of Phaleria macrocarpa (Scheff.) Boerl.
fruits.
It was found to inhibit cell proliferation in HT-29 human colon carcinoma cell line but caused little damage to WRL-68 normal human liver and MRC-5 normal human fibroblast lung cell lines.
The compound was found to sharply affect the viability of HT-29 cells in a dose- and time-dependent manner.
HT-29 cells treated with the compound showed morphological changes under microscopic examination such as cell shrinkage, membrane blebbing, DNA fragmentation, and the occurrence of apoptotic nuclei.
The percentage of early apoptotic, late apoptotic, and dead or necrotic cells was determined by flow cytometry using annexin V-FTIC/PI staining.
In addition, flow cytometry showed that, when the HT-29 cells were treated with 115 µM of the compound, it resulted in G0/G1 phase arrest in a time-dependent manner.
Western blot revealed an upregulation of PUMA, Bak, Bcl-2, and Mcl-1 proteins suggesting that the compound induced apoptosis in HT-29 cells by regulating these proteins.
American Psychological Association (APA)
Lay, Ma Ma& Karsani, Saiful Anuar& Abd Malek, Sri Nurestri. 2014. Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-473876
Modern Language Association (MLA)
Lay, Ma Ma…[et al.]. Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-473876
American Medical Association (AMA)
Lay, Ma Ma& Karsani, Saiful Anuar& Abd Malek, Sri Nurestri. Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-473876
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473876