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Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12
Joint Authors
Chan, Oliver Y. W.
Keng, Bryan M. H.
Heng, Sean S. J.
Ling, Maurice H. T.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The expressions of reference genes used in gene expression studies are assumed to be stable under most circumstances.
However, a number of studies had demonstrated that such genes were found to vary under experimental conditions.
In addition, genes that are stably expressed in an organ may not be stably expressed in other organs or other organisms, suggesting the need to identify reference genes for each organ and organism.
This study aims at identifying stably expressed genes in Escherichia coli.
Microarray datasets from E.
coli substrain MG1655 and 1 dataset from W3110 were analysed.
Coefficient of variance (COV) of was calculated and 10% of the lowest COV from 4631 genes common in the 3 MG1655 sets were analysed using NormFinder.
Glucan biosynthesis protein G (mdoG), which is involved in cell wall synthesis, displayed the lowest weighted COV and weighted NormFinder Stability Index for the MG1655 datasets, while also showing to be the most stable in the dataset for substrain W3110, suggesting that mdoG is a suitable reference gene for E.
coli K-12.
Gene ontology over-representation analysis on the 39 genes suggested an over-representation of cell division, carbohydrate metabolism, and protein synthesis which supports the short generation time of E.
coli.
American Psychological Association (APA)
Heng, Sean S. J.& Chan, Oliver Y. W.& Keng, Bryan M. H.& Ling, Maurice H. T.. 2011. Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12. ISRN Microbiology،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-473938
Modern Language Association (MLA)
Heng, Sean S. J.…[et al.]. Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12. ISRN Microbiology No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-473938
American Medical Association (AMA)
Heng, Sean S. J.& Chan, Oliver Y. W.& Keng, Bryan M. H.& Ling, Maurice H. T.. Glucan Biosynthesis Protein G Is a Suitable Reference Gene in Escherichia coli K-12. ISRN Microbiology. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-473938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-473938