Research on the Interaction between Tubeimoside 1 and HepG2 Cells Using the Microscopic Imaging and Fluorescent Spectra Method
Joint Authors
Lin, Xiaogang
Peng, Wenbing
Liu, Xiaozhu
Li, Wenchao
Zhu, Hao
Rong, Jie
Ye, Changbin
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The treatment of cancer draws interest from researchers worldwide.
Of the different extracts from traditional Chinese medicines, Tubeimoside 1 (TBMS 1) is regarded as an effective treatment for cancer.
To determine the mechanism of TBMS 1, the shape/pattern of HepG2 cells based on the microscopic imaging technology was determined to analyze experimental results; then the fluorescent spectra method was designed to investigate whether TBMS 1 affected HepG2 cells.
A three-dimensional (3D) fluorescent spectra sweep was performed to determine the characteristic wave peak of HepG2 cells.
A 2D fluorescent spectra method was then used to show the florescence change in HepG2 cells following treatment with TBMS 1.
Finally, flow cytometry was employed to analyze the cell cycle of HepG2 cells.
It was shown that TBMS 1 accelerated the death of HepG2 cells and had a strong dose- and time-dependent growth inhibitory effect on HepG2 cells, especially at the G2/M phase.
These results indicate that the fluorescent spectra method is a promising substitute for flow cytometry as it is rapid and cost-effective in HepG2 cells.
American Psychological Association (APA)
Lin, Xiaogang& Li, Wenchao& Ye, Changbin& Liu, Xiaozhu& Zhu, Hao& Peng, Wenbing…[et al.]. 2014. Research on the Interaction between Tubeimoside 1 and HepG2 Cells Using the Microscopic Imaging and Fluorescent Spectra Method. Computational and Mathematical Methods in Medicine،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-474061
Modern Language Association (MLA)
Lin, Xiaogang…[et al.]. Research on the Interaction between Tubeimoside 1 and HepG2 Cells Using the Microscopic Imaging and Fluorescent Spectra Method. Computational and Mathematical Methods in Medicine No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-474061
American Medical Association (AMA)
Lin, Xiaogang& Li, Wenchao& Ye, Changbin& Liu, Xiaozhu& Zhu, Hao& Peng, Wenbing…[et al.]. Research on the Interaction between Tubeimoside 1 and HepG2 Cells Using the Microscopic Imaging and Fluorescent Spectra Method. Computational and Mathematical Methods in Medicine. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-474061
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474061