Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus
Joint Authors
Chiang, Wei-Wei
Chao, Mei
Chuang, Ching-Kai
Chen, Wei-June
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-22
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Japanese encephalitis virus (JEV) is one of approximately 70 flaviviruses, frequently causing symptoms involving the central nervous system.
Mutations of its genomic RNA frequently occur during viral replication, which is believed to be a force contributing to viral evolution.
Nevertheless, accumulating evidences show that some JEV strains may have actually arisen from RNA recombination between genetically different populations of the virus.
We have demonstrated that RNA recombination in JEV occurs unequally in different cell types.
In the present study, viral RNA fragments transfected into as well as viral RNAs synthesized in mosquito cells were shown not to be stable, especially in the early phase of infection possibly via cleavage by exoribonuclease.
Such cleaved small RNA fragments may be further degraded through an RNA interference pathway triggered by viral double-stranded RNA during replication in mosquito cells, resulting in a lower frequency of RNA recombination in mosquito cells compared to that which occurs in mammalian cells.
In fact, adjustment of viral RNA to an appropriately lower level in mosquito cells prevents overgrowth of the virus and is beneficial for cells to survive the infection.
Our findings may also account for the slower evolution of arboviruses as reported previously.
American Psychological Association (APA)
Chiang, Wei-Wei& Chuang, Ching-Kai& Chao, Mei& Chen, Wei-June. 2014. Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-474123
Modern Language Association (MLA)
Chiang, Wei-Wei…[et al.]. Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-474123
American Medical Association (AMA)
Chiang, Wei-Wei& Chuang, Ching-Kai& Chao, Mei& Chen, Wei-June. Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-474123
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474123