Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging
Joint Authors
Ohtomo, Kuni
Watanabe, Makoto
Tojo, Arinobu
Kiryu, Shigeru
Inoue, Yusuke
Source
International Journal of Biomedical Imaging
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The peak signal or the signal at a predetermined, fixed time point after D-luciferin injection may be used for the quantitative analysis of in vivo bioluminescence imaging.
We repeatedly performed sequential bioluminescence imaging after subcutaneous injection of D-luciferin in mice bearing subcutaneous tumors.
The peak time in each measurement became shorter early after cell inoculation, presumably due to gradual establishment of intratumoral vasculature, and reached a plateau of about 10 min on day 10.
Although the correlation between the signal at a fixed time point and the peak signal was high, the signal at 5 or 10 min normalized for the peak signal was lower for earlier days, which caused overestimation of tumor growth.
The time course of the signals after D-luciferin injection may vary with time after cell inoculation, and this variation should be considered when determining the imaging protocol for quantitative bioluminescence tumor monitoring.
American Psychological Association (APA)
Inoue, Yusuke& Kiryu, Shigeru& Watanabe, Makoto& Tojo, Arinobu& Ohtomo, Kuni. 2010. Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging. International Journal of Biomedical Imaging،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-474132
Modern Language Association (MLA)
Inoue, Yusuke…[et al.]. Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging. International Journal of Biomedical Imaging No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-474132
American Medical Association (AMA)
Inoue, Yusuke& Kiryu, Shigeru& Watanabe, Makoto& Tojo, Arinobu& Ohtomo, Kuni. Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging. International Journal of Biomedical Imaging. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-474132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474132