![](/images/graphics-bg.png)
Synergistic Modulation of Cellular Contractility by Mixed Extracellular Matrices
Joint Authors
Source
International Journal of Cell Biology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-29
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The extracellular matrix (ECM) is known to provide various physicochemical cues in directing cell behavior including composition, topography, and dimensionality.
Physical remodeling of the ECM has been documented in a variety of cancers.
In breast cancer, the increased deposition of matrix proteins, their crosslinking, and alignment create a stiffer microenvironment that activates cell contractility and promotes cancer invasion.
In this paper, we sought to study the collective influence of ECM composition and density on the contractile mechanics of human MDA-MB-231 cells making use of the recently established trypsin deadhesion assay.
Using collagen and fibronectin-coated surfaces of varying density, we show that cell contractility is tuned in a density-dependent manner, with faster deadhesion on fibronectin-coated surfaces compared to collagen-coated surfaces under identical coating densities.
The deadhesion responses are significantly delayed when cells are treated with the myosin inhibitor blebbistatin.
By combining collagen and fibronectin at two different densities, we show that mixed ligand surfaces synergistically modulate cell contractility.
Finally, we show that on fibroblast-derived 3D matrices that closely mimic in vivo matrices, cells are strongly polarized and exhibit faster deadhesion compared to the mixed ligand surfaces.
Together, our results demonstrate that ECM composition, density, and 3D organization collectively regulate cell contractility.
American Psychological Association (APA)
Kapoor, Aastha& Sen, Shamik. 2012. Synergistic Modulation of Cellular Contractility by Mixed Extracellular Matrices. International Journal of Cell Biology،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-474147
Modern Language Association (MLA)
Kapoor, Aastha& Sen, Shamik. Synergistic Modulation of Cellular Contractility by Mixed Extracellular Matrices. International Journal of Cell Biology No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-474147
American Medical Association (AMA)
Kapoor, Aastha& Sen, Shamik. Synergistic Modulation of Cellular Contractility by Mixed Extracellular Matrices. International Journal of Cell Biology. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-474147
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474147