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In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera
Joint Authors
Ardalan, Bach
Nathanson, Lubov
Lokeshwar, Balakrishna L.
Sarkar, Malancha
Doshi, Nikesh
Subbarayan, Pochi R.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Achyranthes aspera (family Amaranthaceae) is known for its anticancer properties.
We have systematically validated the in vitro and in vivo anticancer properties of this plant.
However, we do not know its mode of action.
Global gene expression analyses may help decipher its mode of action.
In the absence of identified active molecules, we believe this is the best approach to discover the mode of action of natural products with known medicinal properties.
We exposed human pancreatic cancer cell line MiaPaCa-2 (CRL-1420) to 34 μg/mL of LE for 24, 48, and 72 hours.
Gene expression analyses were performed using whole human genome microarrays (Agilent Technologies, USA).
In our analyses, 82 (54/28) genes passed the quality control parameter, set at FDR ≤ 0.01 and FC of ≥±2.
LE predominantly affected pathways of immune response, metabolism, development, gene expression regulation, cell adhesion, cystic fibrosis transmembrane conductance regulation (CFTR), and chemotaxis (MetaCore tool (Thomson Reuters, NY)).
Disease biomarker enrichment analysis identified LE regulated genes involved in Vasculitis—inflammation of blood vessels.
Arthritis and pancreatitis are two of many etiologies for vasculitis.
The outcome of disease network analysis supports the medicinal use of A.
aspera, viz, to stop bleeding, as a cure for pancreatic cancer, as an antiarthritic medication, and so forth.
American Psychological Association (APA)
Subbarayan, Pochi R.& Sarkar, Malancha& Nathanson, Lubov& Doshi, Nikesh& Lokeshwar, Balakrishna L.& Ardalan, Bach. 2013. In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-474164
Modern Language Association (MLA)
Subbarayan, Pochi R.…[et al.]. In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-474164
American Medical Association (AMA)
Subbarayan, Pochi R.& Sarkar, Malancha& Nathanson, Lubov& Doshi, Nikesh& Lokeshwar, Balakrishna L.& Ardalan, Bach. In Vitro Global Gene Expression Analyses Support the Ethnopharmacological Use of Achyranthes aspera. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-474164
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474164