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Triplet Analysis That Identifies Unpaired Regions of Functional RNAs
Joint Authors
Sugimoto, Naoki
Yamaguchi, Yoshie
Kawakami, Junji
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-19
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
We developed a novel method for analyzing RNA sequences, deemed triplet analysis, and applied the method in an in vitro RNA selection experiment in which HIV-1 Tat was the target.
Aptamers are nucleic acids that bind a desired target (bait), and to date, many aptamers have been identified by in vitro selection from enough concentrated libraries in which many RNAs had an obvious consensus primary sequence after sufficient cycles of the selection.
Therefore, the higher-order structural features of the aptamers that are indispensable for interaction with the bait must be determined by additional investigation of the aptamers.
In contrast, our triplet analysis enabled us to extract important information on functional primary and secondary structure from minimally concentrated RNA libraries.
As a result, by using our method, an important unpaired region that is similar to the bulge of TAR was readily predicted from a partially concentrated library in which no consensus sequence was revealed by a conventional sequence analysis.
Moreover, our analysis method may be used to assess a variety of structural motifs with desired function.
American Psychological Association (APA)
Kawakami, Junji& Yamaguchi, Yoshie& Sugimoto, Naoki. 2011. Triplet Analysis That Identifies Unpaired Regions of Functional RNAs. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-474179
Modern Language Association (MLA)
Kawakami, Junji…[et al.]. Triplet Analysis That Identifies Unpaired Regions of Functional RNAs. Journal of Nucleic Acids No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-474179
American Medical Association (AMA)
Kawakami, Junji& Yamaguchi, Yoshie& Sugimoto, Naoki. Triplet Analysis That Identifies Unpaired Regions of Functional RNAs. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-474179
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474179