Viral Bacterial Artificial Chromosomes : Generation, Mutagenesis, and Removal of Mini-F Sequences
Joint Authors
Kaufer, Benedikt B.
Tischer, B. Karsten
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-23
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Maintenance and manipulation of large DNA and RNA virus genomes had presented an obstacle for virological research.
BAC vectors provided a solution to both problems as they can harbor large DNA sequences and can efficiently be modified using well-established mutagenesis techniques in Escherichia coli.
Numerous DNA virus genomes of herpesvirus and pox virus were cloned into mini-F vectors.
In addition, several reverse genetic systems for RNA viruses such as members of Coronaviridae and Flaviviridae could be established based on BAC constructs.
Transfection into susceptible eukaryotic cells of virus DNA cloned as a BAC allows reconstitution of recombinant viruses.
In this paper, we provide an overview on the strategies that can be used for the generation of virus BAC vectors and also on systems that are currently available for various virus species.
Furthermore, we address common mutagenesis techniques that allow modification of BACs from single-nucleotide substitutions to deletion of viral genes or insertion of foreign sequences.
Finally, we review the reconstitution of viruses from BAC vectors and the removal of the bacterial sequences from the virus genome during this process.
American Psychological Association (APA)
Tischer, B. Karsten& Kaufer, Benedikt B.. 2012. Viral Bacterial Artificial Chromosomes : Generation, Mutagenesis, and Removal of Mini-F Sequences. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-474241
Modern Language Association (MLA)
Tischer, B. Karsten& Kaufer, Benedikt B.. Viral Bacterial Artificial Chromosomes : Generation, Mutagenesis, and Removal of Mini-F Sequences. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-474241
American Medical Association (AMA)
Tischer, B. Karsten& Kaufer, Benedikt B.. Viral Bacterial Artificial Chromosomes : Generation, Mutagenesis, and Removal of Mini-F Sequences. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-474241
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474241