Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays
Joint Authors
Nadler, Timothy
Cappione, Amedeo
Mabuchi, Masaharu
Smith, Janet
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Expression profiling on a large scale, as is the case in drug discovery, is often accomplished through use of sophisticated solid-phase protein microarrays or multiplex bead technologies.
While offering both high-throughput and high-content analysis, these platforms are often too cost prohibitive or technically challenging for many research settings.
Capitalizing on the favorable attributes of the standard ELISA and slot blotting techniques, we developed a modified dot blot assay that provides a simple cost-effective alternative for semiquantitative expression analysis of multiple proteins across multiple samples.
Similar in protocol to an ELISA, but based in a membrane bound 96-well microplate, the assay takes advantage of vacuum filtration to expedite the tedious process of washing in between binding steps.
We report on the optimization of the assay and demonstrate its use in profiling temporal changes in phosphorylation events in the well-characterized EGF-induced signaling cascade of A431 cells.
American Psychological Association (APA)
Cappione, Amedeo& Smith, Janet& Mabuchi, Masaharu& Nadler, Timothy. 2012. Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-474374
Modern Language Association (MLA)
Cappione, Amedeo…[et al.]. Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays. International Journal of Proteomics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-474374
American Medical Association (AMA)
Cappione, Amedeo& Smith, Janet& Mabuchi, Masaharu& Nadler, Timothy. Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-474374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474374