Age Modulates Fe3O4 Nanoparticles Liver Toxicity : Dose-Dependent Decrease in Mitochondrial Respiratory Chain Complexes Activities and Coupling in Middle-Aged as Compared to Young Rats
Joint Authors
Ben Tahar, Lotfi
Vogel, Thomas
Tebourbi, Olfa
Wolff, Valérie
Abdelmelek, Hafedh
Zoll, Joffrey
Geny, Bernard
Sakly, Mohsen
Smiri, Leila
Baratli, Yosra
Charles, Anne-Laure
Bouitbir, Jamal
Auger, Cyril
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We examined the effects of iron oxide nanoparticles (IONPs) on mitochondrial respiratory chain complexes activities and mitochondrial coupling in young (3 months) and middle-aged (18 months) rat liver, organ largely involved in body iron detoxification.
Isolated liver mitochondria were extracted using differential centrifugations.
Maximal oxidative capacities (Vmax, complexes I, III, and IV activities), Vsucc (complexes II, III, and IV activities), and Vtmpd, (complex IV activity), together with mitochondrial coupling (Vmax/V0) were determined in controls conditions and after exposure to 250, 300, and 350 μg/ml Fe3O4 in young and middle-aged rats.
In young liver mitochondria, exposure to IONPs did not alter mitochondrial function.
In contrast, IONPs dose-dependently impaired all complexes of the mitochondrial respiratory chain in middle-aged rat liver: Vmax (from 30 ± 1.6 to 17.9 ± 1.5; P<0.001), Vsucc (from 33.9 ± 1.7 to 24.3 ± 1.0; P<0.01), Vtmpd (from 43.0 ± 1.6 to 26.3 ± 2.2 µmol O2/min/g protein; P<0.001) using Fe3O4 350 µg/ml.
Mitochondrial coupling also decreased.
Interestingly, 350 μg/ml Fe3O4 in the form of Fe3+ solution did not impair liver mitochondrial function in middle-aged rats.
Thus, IONPs showed a specific toxicity in middle-aged rats suggesting caution when using it in old age.
American Psychological Association (APA)
Baratli, Yosra& Charles, Anne-Laure& Wolff, Valérie& Ben Tahar, Lotfi& Smiri, Leila& Bouitbir, Jamal…[et al.]. 2014. Age Modulates Fe3O4 Nanoparticles Liver Toxicity : Dose-Dependent Decrease in Mitochondrial Respiratory Chain Complexes Activities and Coupling in Middle-Aged as Compared to Young Rats. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-474408
Modern Language Association (MLA)
Baratli, Yosra…[et al.]. Age Modulates Fe3O4 Nanoparticles Liver Toxicity : Dose-Dependent Decrease in Mitochondrial Respiratory Chain Complexes Activities and Coupling in Middle-Aged as Compared to Young Rats. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-474408
American Medical Association (AMA)
Baratli, Yosra& Charles, Anne-Laure& Wolff, Valérie& Ben Tahar, Lotfi& Smiri, Leila& Bouitbir, Jamal…[et al.]. Age Modulates Fe3O4 Nanoparticles Liver Toxicity : Dose-Dependent Decrease in Mitochondrial Respiratory Chain Complexes Activities and Coupling in Middle-Aged as Compared to Young Rats. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-474408
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474408