Molecular Characterization of Lipopolysaccharide Binding to Human α-1-Acid Glycoprotein
Joint Authors
Cooper, Matthew A.
Azad, Mohammad A. K.
Velkov, Tony
Li, Jian
Yuriev, Elizabeth
Huang, Johnny X.
Nation, Roger L.
Baker, Mark A.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-20
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The ability of AGP to bind circulating lipopolysaccharide (LPS) in plasma is believed to help reduce the proinflammatory effect of bacterial lipid A molecules.
Here, for the first time we have characterized human AGP binding characteristics of the LPS from a number of pathogenic Gram-negative bacteria: Escherichia coli, Salmonella typhimurium, Klebsiella pneumonia, Pseudomonas aeruginosa, and Serratia marcescens.
The binding affinity and structure activity relationships (SAR) of the AGP-LPS interactions were characterized by surface plasma resonance (SPR).
In order to dissect the contribution of the lipid A, core oligosaccharide and O-antigen polysaccharide components of LPS, the AGP binding affinity of LPS from smooth strains, were compared to lipid A, Kdo2-lipid A, Ra, Rd, and Re rough LPS mutants.
The SAR analysis enabled by the binding data suggested that, in addition to the important role played by the lipid A and core components of LPS, it is predominately the unique species- and strain-specific carbohydrate structure of the O-antigen polysaccharide that largely determines the binding affinity for AGP.
Together, these data are consistent with the role of AGP in the binding and transport of LPS in plasma during acute-phase inflammatory responses to invading Gram-negative bacteria.
American Psychological Association (APA)
Huang, Johnny X.& Azad, Mohammad A. K.& Yuriev, Elizabeth& Baker, Mark A.& Nation, Roger L.& Li, Jian…[et al.]. 2012. Molecular Characterization of Lipopolysaccharide Binding to Human α-1-Acid Glycoprotein. Journal of Lipids،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-474497
Modern Language Association (MLA)
Huang, Johnny X.…[et al.]. Molecular Characterization of Lipopolysaccharide Binding to Human α-1-Acid Glycoprotein. Journal of Lipids No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-474497
American Medical Association (AMA)
Huang, Johnny X.& Azad, Mohammad A. K.& Yuriev, Elizabeth& Baker, Mark A.& Nation, Roger L.& Li, Jian…[et al.]. Molecular Characterization of Lipopolysaccharide Binding to Human α-1-Acid Glycoprotein. Journal of Lipids. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-474497
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474497