Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis
Joint Authors
Ondřej, Vladan
Skálová, Dagmar
Lebeda, Aleš
Doležalová, Ivana
Navrátilová, Božena
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C.
sativus), x=7; muskmelon (C.
melo) and wild Cucumis species, x=12).
Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers.
The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants).
Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin.
Ploidy level was estimated by flow cytometry.
Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared.
Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts.
Nevertheless, the hybrid character of putative hybrid accessions obtained after cross in vivo and in vitro pollination was not confirmed.
American Psychological Association (APA)
Skálová, Dagmar& Ondřej, Vladan& Doležalová, Ivana& Navrátilová, Božena& Lebeda, Aleš. 2010. Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-474528
Modern Language Association (MLA)
Skálová, Dagmar…[et al.]. Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-474528
American Medical Association (AMA)
Skálová, Dagmar& Ondřej, Vladan& Doležalová, Ivana& Navrátilová, Božena& Lebeda, Aleš. Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-474528
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474528