B Epitope Multiplicity and BT Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines
Joint Authors
Andreu, David
Cubillos, Carolina
de la Torre, Beatriz G.
Moreno, Noelia
Sobrino, Francisco
Bárcena, Juan
Blanco, Esther
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Synthetic peptides incorporating protective B- and T-cell epitopes are candidates for new safer foot-and-mouth disease (FMD) vaccines.
We have reported that dendrimeric peptides including four copies of a B-cell epitope (VP1 136 to 154) linked to a T-cell epitope (3A 21 to 35) of FMD virus (FMDV) elicit potent B- and T-cell specific responses and confer protection to viral challenge, while juxtaposition of these epitopes in a linear peptide induces less efficient responses.
To assess the relevance of B-cell epitope multivalency, dendrimers bearing two (B2T) or four (B4T) copies of the B-cell epitope from type O FMDV (a widespread circulating serotype) were tested in CD1 mice and showed that multivalency is advantageous over simple B-T-epitope juxtaposition, resulting in efficient induction of neutralizing antibodies and optimal release of IFNγ.
Interestingly, the bivalent B2T construction elicited similar or even better B- and T-cell specific responses than tetravalent B4T.
In addition, the presence of the T-cell epitope and its orientation were shown to be critical for the immunogenicity of the linear juxtaposed monovalent peptides analyzed in parallel.
Taken together, our results provide useful insights for a more accurate design of FMD subunit vaccines.
American Psychological Association (APA)
Blanco, Esther& Cubillos, Carolina& Moreno, Noelia& Bárcena, Juan& de la Torre, Beatriz G.& Andreu, David…[et al.]. 2013. B Epitope Multiplicity and BT Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-474574
Modern Language Association (MLA)
Blanco, Esther…[et al.]. B Epitope Multiplicity and BT Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines. Clinical and Developmental Immunology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-474574
American Medical Association (AMA)
Blanco, Esther& Cubillos, Carolina& Moreno, Noelia& Bárcena, Juan& de la Torre, Beatriz G.& Andreu, David…[et al.]. B Epitope Multiplicity and BT Epitope Orientation Influence Immunogenicity of Foot-and-Mouth Disease Peptide Vaccines. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-474574
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474574