Fluorescence Intensity Normalisation : Correcting for Time Effects in Large-Scale Flow Cytometric Analysis
Joint Authors
Plagnol, Vincent
Dendrou, Calliope A.
Fung, Erik
Esposito, Laura
Wicker, Linda S.
Todd, John A.
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-17
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
A next step to interpret the findings generated by genome-wide association studies is to associate molecular quantitative traits with disease-associated alleles.
To this end, researchers are linking disease risk alleles with gene expression quantitative trait loci (eQTL).
However, gene expression at the mRNA level is only an intermediate trait and flow cytometry analysis can provide more downstream and biologically valuable protein level information in multiple cell subsets simultaneously using freshly obtained samples.
Because the throughput of flow cytometry is currently limited, experiments may need to span over several weeks or months to obtain a sufficient sample size to demonstrate genetic association.
Therefore, normalisation methods are needed to control for technical variability and compare flow cytometry data over an extended period of time.
We show how the use of normalising fluorospheres improves the repeatability of a cell surface CD25-APC mean fluorescence intensity phenotype on CD4+ memory T cells.
We investigate two types of normalising beads: broad spectrum and spectrum matched.
Lastly, we propose two alternative normalisation procedures that are usable in the absence of normalising beads.
American Psychological Association (APA)
Dendrou, Calliope A.& Fung, Erik& Esposito, Laura& Todd, John A.& Wicker, Linda S.& Plagnol, Vincent. 2009. Fluorescence Intensity Normalisation : Correcting for Time Effects in Large-Scale Flow Cytometric Analysis. Advances in Bioinformatics،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-474592
Modern Language Association (MLA)
Dendrou, Calliope A.…[et al.]. Fluorescence Intensity Normalisation : Correcting for Time Effects in Large-Scale Flow Cytometric Analysis. Advances in Bioinformatics No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-474592
American Medical Association (AMA)
Dendrou, Calliope A.& Fung, Erik& Esposito, Laura& Todd, John A.& Wicker, Linda S.& Plagnol, Vincent. Fluorescence Intensity Normalisation : Correcting for Time Effects in Large-Scale Flow Cytometric Analysis. Advances in Bioinformatics. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-474592
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474592