In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Joint Authors
Nakashima, Yoshihiro
Uekama, Kaneto
Aramaki, Yukihiko
Arima, Hidetoshi
Motoyama, Keiichi
Hirayama, Fumitoshi
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector.
Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed the highest gene transfer activity in HepG2 cells without any significant cytotoxicity.
In addition, γ-CyD enhanced the encapsulation ratio of pDNA with AF-liposomes, and also increased gene transfer activity as the entrapment ratio of pDNA into AF-liposomes was increased.
γ-CyD stabilized the liposomal membrane of AF-liposomes and inhibited the release of calcein from AF-liposomes.
The stabilizing effect of γ-CyD may be, at least in part, involved in the enhancing gene transfer activity of pDNA/γ-CyD/AF-liposomes.
Therefore, these results suggest the potential use of γ-CyD for an enhancer of transfection efficiency of AF-liposomes.
American Psychological Association (APA)
Motoyama, Keiichi& Nakashima, Yoshihiro& Aramaki, Yukihiko& Hirayama, Fumitoshi& Uekama, Kaneto& Arima, Hidetoshi. 2010. In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes. Journal of Drug Delivery،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-474593
Modern Language Association (MLA)
Motoyama, Keiichi…[et al.]. In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes. Journal of Drug Delivery No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-474593
American Medical Association (AMA)
Motoyama, Keiichi& Nakashima, Yoshihiro& Aramaki, Yukihiko& Hirayama, Fumitoshi& Uekama, Kaneto& Arima, Hidetoshi. In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes. Journal of Drug Delivery. 2010. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-474593
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474593