A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice
Joint Authors
Wang, Ting
Yi, Jun
Yuan, Shi-fang
Fan, Jing
Lv, Yong-gang
Chen, Jiang-hao
Ling, Rui
Li, Jun-tang
Wang, Hui
Li, Nan-lin
Wang, Yu-cai
Wang, Ling
Yao, Qing
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-27
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Anesthetic isoflurane (ISO) has immunomodulatory effects.
In the present study, we investigated whether a subanesthetic dose of ISO (0.7%) protected against zymosan (ZY) induced inflammatory responses in the murine lung and isolated neutrophils.
At 1 and 6 hrs after ZY administration intraperitoneally, ISO was inhaled for 1 hr, and 24 hrs later, lung inflammation and injury were assessed.
We found that ISO improved the survival rate of mice and mitigated lung injury as characterized by the histopathology, wet-to-dry weight ratio, protein leakage, and lung function index.
ISO significantly attenuated ZY-induced lung neutrophil recruitment and inflammation.
This was suggested by the downregulation of (a) endothelial adhesion molecule expression and myeloperoxidase (MPO) activity in lung tissue and polymorphonuclear neutrophils (b) chemokines, and (c) proinflammatory cytokines in BALF.
Furthermore, ZY-induced nuclear translocation and DNA-binding activity of NF-κB p65 were also reduced by ISO.
ISO treatment inhibited iNOS expression and activity, as well as subsequent nitric oxide generation.
Consistent with these in vivo observations, in vitro studies confirmed that ISO blocked NF-κB and iNOS activation in primary mouse neutrophils challenged by ZY.
These results provide evidence that 0.7% ISO ameliorates inflammatory responses in ZY-treated mouse lung and primary neutrophils.
American Psychological Association (APA)
Wang, Hui& Fan, Jing& Li, Nan-lin& Li, Jun-tang& Yuan, Shi-fang& Yi, Jun…[et al.]. 2013. A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-474831
Modern Language Association (MLA)
Wang, Hui…[et al.]. A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice. Mediators of Inflammation No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-474831
American Medical Association (AMA)
Wang, Hui& Fan, Jing& Li, Nan-lin& Li, Jun-tang& Yuan, Shi-fang& Yi, Jun…[et al.]. A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-474831
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474831