Two Major Archaeal Pseudomurein Endoisopeptidases : PeiW and PeiP
Joint Authors
Kok, Jan
Visweswaran, Ganesh Ram R.
Dijkstra, Bauke W.
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-11
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
PeiW (UniProtKB Q7LYX0) and PeiP (UniProtKB Q77WJ4) are the two major pseudomurein endoisopeptidases (Pei) that are known to cleave pseudomurein cell-wall sacculi of the members of the methanogenic orders Methanobacteriales and Methanopyrales.
Both enzymes, originating from prophages specific for some methanogenic archaeal species, hydrolyze the ϵ(Ala)-Lys bond of the peptide linker between adjacent pseudomurein layers.
Because lysozyme is not able to cleave the pseudomurein cell wall, the enzymes are used in protoplast preparation and in DNA isolation from pseudomurein cell-wall-containing methanogens.
Moreover, PeiW increases the probe permeability ratio and enables fluorescence in situ hybridization (FISH) and catalyzed reporter deposition (CARD-) FISH experiments to be performed on these methanogens.
American Psychological Association (APA)
Visweswaran, Ganesh Ram R.& Dijkstra, Bauke W.& Kok, Jan. 2010. Two Major Archaeal Pseudomurein Endoisopeptidases : PeiW and PeiP. Archaea،Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-474896
Modern Language Association (MLA)
Visweswaran, Ganesh Ram R.…[et al.]. Two Major Archaeal Pseudomurein Endoisopeptidases : PeiW and PeiP. Archaea No. 2010 (2010), pp.1-4.
https://search.emarefa.net/detail/BIM-474896
American Medical Association (AMA)
Visweswaran, Ganesh Ram R.& Dijkstra, Bauke W.& Kok, Jan. Two Major Archaeal Pseudomurein Endoisopeptidases : PeiW and PeiP. Archaea. 2010. Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-474896
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474896