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Transcriptional Profile and Structural Conservation of SUMO-Specific Proteases in Schistosoma mansoni
Joint Authors
Pereira, Roberta Verciano
Castro-Borges, William
Cabral, Fernanda Janku
Jannotti-Passos, Liana Konovaloff
Guerra-Sá, Renata
de Souza Gomes, Matheus
Source
Journal of Parasitology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Small ubiquitin-related modifier (SUMO) is involved in numerous cellular processes including protein localization, transcription, and cell cycle control.
SUMOylation is a dynamic process, catalyzed by three SUMO-specific enzymes and reversed by Sentrin/SUMO-specific proteases (SENPs).
Here we report the characterization of these proteases in Schistosoma mansoni.
Using in silico analysis, we identified two SENPs sequences, orthologs of mammalian SENP1 and SENP7, confirming their identities and conservation through phylogenetic analysis.
In addition, the transcript levels of Smsenp1/7 in cercariae, adult worms, and in vitro cultivated schistosomula were measured by qRT-PCR.
Our data revealed upregulation of the Smsenp1/7 transcripts in cercariae and early schistosomula, followed by a marked differential gene expression in the other analyzed stages.
However, no significant difference in expression profile between the paralogs was observed for the analyzed stages.
Furthermore, in order to detect deSUMOylating capabilities in crude parasite extracts, SmSENP1 enzymatic activity was evaluated using SUMO-1-AMC substrate.
The endopeptidase activity related to SUMO-1 precursor processing did not differ significantly between cercariae and adult worms.
Taken together, these results support the developmentally regulated expression of SUMO-specific proteases in S.
mansoni.
American Psychological Association (APA)
Pereira, Roberta Verciano& Cabral, Fernanda Janku& de Souza Gomes, Matheus& Jannotti-Passos, Liana Konovaloff& Castro-Borges, William& Guerra-Sá, Renata. 2012. Transcriptional Profile and Structural Conservation of SUMO-Specific Proteases in Schistosoma mansoni. Journal of Parasitology Research،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-474941
Modern Language Association (MLA)
Pereira, Roberta Verciano…[et al.]. Transcriptional Profile and Structural Conservation of SUMO-Specific Proteases in Schistosoma mansoni. Journal of Parasitology Research No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-474941
American Medical Association (AMA)
Pereira, Roberta Verciano& Cabral, Fernanda Janku& de Souza Gomes, Matheus& Jannotti-Passos, Liana Konovaloff& Castro-Borges, William& Guerra-Sá, Renata. Transcriptional Profile and Structural Conservation of SUMO-Specific Proteases in Schistosoma mansoni. Journal of Parasitology Research. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-474941
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474941