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ICAM-1 Upregulation in Ethanol-Induced Fatty Murine Livers Promotes Injury and Sinusoidal Leukocyte Adherence after Transplantation
Joint Authors
Ramshesh, Venkat K.
Karrasch, Thomas
Zhong, Zhi
Theruvath, Tom P.
Currin, Robert T.
Lemasters, John J.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Transplantation of ethanol-induced steatotic livers causes increased graft injury.
We hypothesized that upregulation of hepatic ICAM-1 after ethanol produces increased leukocyte adherence, resulting in increased generation of reactive oxygen species (ROS) and injury after liver transplantation (LT).
Methods.
C57BL/6 wildtype (WT) and ICAM-1 knockout (KO) mice were gavaged with ethanol (6 g/kg) or water.
LT was then performed into WT recipients.
Necrosis and apoptosis, 4-hydroxynonenal (4-HNE) immunostaining, and sinusoidal leukocyte movement by intravital microscopy were assessed.
Results.
Ethanol gavage of WT mice increased hepatic triglycerides 10-fold compared to water treatment (P<0.05).
ICAM-1 also increased, but ALT was normal.
At 8 h after LT of WT grafts, ALT increased 2-fold more with ethanol than water treatment (P<0.05).
Compared to ethanol-treated WT grafts, ALT from ethanol-treated KO grafts was 78% less (P<0.05).
Apoptosis also decreased by 75% (P<0.05), and 4-HNE staining after LT was also decreased in ethanol-treated KO grafts compared to WT.
Intravital microscopy demonstrated a 2-fold decrease in leukocyte adhesion in KO grafts compared to WT grafts.
Conclusions.
Increased ICAM-1 expression in ethanol-treated fatty livers predisposes to leukocyte adherence after LT, which leads to a disturbed microcirculation, oxidative stress and graft injury.
American Psychological Association (APA)
Theruvath, Tom P.& Ramshesh, Venkat K.& Zhong, Zhi& Currin, Robert T.& Karrasch, Thomas& Lemasters, John J.. 2012. ICAM-1 Upregulation in Ethanol-Induced Fatty Murine Livers Promotes Injury and Sinusoidal Leukocyte Adherence after Transplantation. HPB Surgery،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-474946
Modern Language Association (MLA)
Theruvath, Tom P.…[et al.]. ICAM-1 Upregulation in Ethanol-Induced Fatty Murine Livers Promotes Injury and Sinusoidal Leukocyte Adherence after Transplantation. HPB Surgery No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-474946
American Medical Association (AMA)
Theruvath, Tom P.& Ramshesh, Venkat K.& Zhong, Zhi& Currin, Robert T.& Karrasch, Thomas& Lemasters, John J.. ICAM-1 Upregulation in Ethanol-Induced Fatty Murine Livers Promotes Injury and Sinusoidal Leukocyte Adherence after Transplantation. HPB Surgery. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-474946
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-474946