Analysis of Chloramphenicol and Its Related Compound 2-Amino-1-(4-nitrophenyl)propane-1,3-diol by Reversed-Phase High-Performance Liquid Chromatography with UV Detection
Joint Authors
Source
Chromatography Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-08
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A simple and stability-indicating liquid chromatographic method is developed for the analysis of chloramphenicol and its related compound 2-amino-1-(4-nitrophenyl)propane-1,3-diol in two pharmaceutical forms.
Liquid chromatography with a UV detector at a wavelength of 278 nm using a reversed phase C18 stationary phase has been employed in this study.
Isocratic elution is employed using a mixture of sodium pentanesulfonate solution (0.012 M), acetonitrile, and glacial acetic acid (85 : 15 : 1, v/v).
This new method is validated in accordance with USP requirements for new methods for assay determination, which include accuracy, precision, specificity, linearity and range.
This method shows enough selectivity, sensitivity, accuracy, precision, and linearity range to satisfy Federal Drug Administration/International Conference on Harmonization regulatory requirements.
The current method demonstrates good linearity over the range of 0.04–0.16 mg/mL of chloramphenicol.
The accuracy of the method is 100.0% with a relative standard deviation of 0.1%.
The precision of this method reflected by relative standard deviation of replicates is 0.1%.
The method is sensitive with a detection limit of 0.005% for chloramphenicol.
The related substance of chloramphenicol (2-amino-1-(4-nitrophenyl)propane-1,3-diol) can be selectively determined with a good resolution in two pharmaceutical forms: eye ointment and eye drops.
American Psychological Association (APA)
al-Rimawi, Fuad& Kharoaf, Maher. 2011. Analysis of Chloramphenicol and Its Related Compound 2-Amino-1-(4-nitrophenyl)propane-1,3-diol by Reversed-Phase High-Performance Liquid Chromatography with UV Detection. Chromatography Research International،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-475082
Modern Language Association (MLA)
al-Rimawi, Fuad& Kharoaf, Maher. Analysis of Chloramphenicol and Its Related Compound 2-Amino-1-(4-nitrophenyl)propane-1,3-diol by Reversed-Phase High-Performance Liquid Chromatography with UV Detection. Chromatography Research International No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-475082
American Medical Association (AMA)
al-Rimawi, Fuad& Kharoaf, Maher. Analysis of Chloramphenicol and Its Related Compound 2-Amino-1-(4-nitrophenyl)propane-1,3-diol by Reversed-Phase High-Performance Liquid Chromatography with UV Detection. Chromatography Research International. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-475082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475082