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Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
Joint Authors
Cho, In-Cheol
Yu, Seong-Lan
Jin, Dong-Il
Jung, Woo-Young
Lee, Jun-Heon
Jung, Kie-Chul
Lim, Hyun-Tae
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Pigs have been considered as donors for xenotransplantation in the replacement of human organs and tissues.
However, porcine endogenous retroviruses (PERVs) might transmit new infectious disease to humans during xenotransplantation.
To investigate PERV integration sites, 45 PERV-positive BAC clones, including 12 PERV-A, 16 PERV-B, and 17 PERV-C clones, were identified from the NIH miniature pig BAC library.
The analysis of 12 selected full-length sequences of PERVs, including the long terminal repeat (LTR) region, identified the expected of open reading frame length, an indicative of active PERV, in all five PERV-C clones and one of the four PERV-B clones.
Premature stop codons were observed in only three PERV-A clones.
Also, eleven PERV integration sites were mapped using a 5000-rad IMpRH panel.
The map locations of PERV-C clones have not been reported before, thus they are novel PERV clones identified in this study.
The results could provide basic information for the elimination of site-specific PERVs in selection of pigs for xenotransplantation.
American Psychological Association (APA)
Yu, Seong-Lan& Jung, Woo-Young& Jung, Kie-Chul& Cho, In-Cheol& Lim, Hyun-Tae& Jin, Dong-Il…[et al.]. 2011. Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-475112
Modern Language Association (MLA)
Yu, Seong-Lan…[et al.]. Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-475112
American Medical Association (AMA)
Yu, Seong-Lan& Jung, Woo-Young& Jung, Kie-Chul& Cho, In-Cheol& Lim, Hyun-Tae& Jin, Dong-Il…[et al.]. Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library. Journal of Biomedicine and Biotechnology. 2011. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-475112
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475112