A Phylogenetic Index for Cichlid Microsatellite Primers
Joint Authors
Kunkle, Robert D.
Renn, Suzy C. P.
Reilly, Christian R. L.
Source
International Journal of Zoology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-02
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Microsatellites abound in most organisms and have proven useful for a range of genetic and genomic studies.
Once primers have been created, they can be applied to populations or taxa that have diverged from the source taxon.
We use PCR amplification, in a 96-well format, to determine the presence and absence of 46 microsatellite loci in 13 cichlid species.
At least one primer set amplified a product in each species tested, and some products were present in nearly all species.
These results are compared to the known phylogenetic relationships among cichlids.
While we do not address intraspecies variation, our results present a phylogenetic index for the success of microsatellite PCR primer product amplification, thus providing information regarding a collection of primers that are applicable to wide range of species.
Through the use of such a uniform primer panel, the potential impact for cross species would be increased.
American Psychological Association (APA)
Kunkle, Robert D.& Reilly, Christian R. L.& Renn, Suzy C. P.. 2010. A Phylogenetic Index for Cichlid Microsatellite Primers. International Journal of Zoology،Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-475129
Modern Language Association (MLA)
Kunkle, Robert D.…[et al.]. A Phylogenetic Index for Cichlid Microsatellite Primers. International Journal of Zoology No. 2010 (2010), pp.1-5.
https://search.emarefa.net/detail/BIM-475129
American Medical Association (AMA)
Kunkle, Robert D.& Reilly, Christian R. L.& Renn, Suzy C. P.. A Phylogenetic Index for Cichlid Microsatellite Primers. International Journal of Zoology. 2010. Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-475129
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475129