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The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment
Joint Authors
Lunde, Merete
Indahl, Ulf
Snipen, Lars
Vebø, Heidi
Aakra, Ågot
Gjerstad, Øystein
Nes, Ingolf Figved
Source
International Journal of Microbiology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Many Enterococcus faecalis strains display tolerance or resistance to many antibiotics, but genes that contribute to the resistance cannot be specified.
The multiresistant E.
faecalis V583, for which the complete genome sequence is available, survives and grows in media containing relatively high levels of chloramphenicol.
No specific genes coding for chloramphenicol resistance has been recognized in V583.
We used microarrays to identify genes and mechanisms behind the tolerance to chloramphenicol in V583, by comparison of cells treated with subinhibitory concentrations of chloramphenicol and untreated V583 cells.
During a time course experiment, more than 600 genes were significantly differentially transcribed.
Since chloramphenicol affects protein synthesis in bacteria, many genes involved in protein synthesis, for example, genes for ribosomal proteins, were induced.
Genes involved in amino acid biosynthesis, for example, genes for tRNA synthetases and energy metabolism were downregulated, mainly.
Among the upregulated genes were EF1732 and EF1733, which code for potential chloramphenicol transporters.
Efflux of drug out of the cells may be one mechanism used by V583 to overcome the effect of chloramphenicol.
American Psychological Association (APA)
Aakra, Ågot& Vebø, Heidi& Indahl, Ulf& Snipen, Lars& Gjerstad, Øystein& Lunde, Merete…[et al.]. 2010. The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment. International Journal of Microbiology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-475163
Modern Language Association (MLA)
Aakra, Ågot…[et al.]. The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment. International Journal of Microbiology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-475163
American Medical Association (AMA)
Aakra, Ågot& Vebø, Heidi& Indahl, Ulf& Snipen, Lars& Gjerstad, Øystein& Lunde, Merete…[et al.]. The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment. International Journal of Microbiology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-475163
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475163