Helicobacter pylori Is Not Eradicated after Triple Therapy : A Nested PCR Based Study
Joint Authors
Patel, Saurabh Kumar
Nath, Gopal
Jain, Ashok Kumar
Pratap, Chandra Bhan
Mishra, Girish Narayan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-25
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Detection of Helicobacter pylori after triple therapy is usually carried out by either rapid urease test (RUT), urea breath test (UBT), histology, bacterial isolation, and single round PCR or serological tests.
In this study, antral biopsy specimens from 25 patients were tested for H.
pylori by RUT, culture, histology, and nested PCR in their antral biopsy specimens before and after treatment.
Three genes, namely, heat shock protein (hsp60), phosphoglucosamine mutase (ureC), and flagellar export ATP synthase (fliI) of H.
pylori were targeted.
Of the 25 antral biopsy specimens, the RUT, culture, histology, and nested PCR positivity dropped from 81.8% to 12%, 31% to 0%, 100 to 84%, and 100% to 92%, respectively, before and after therapy.
Further, hsp60 specific amplicons from 23 out of 25 patients gave identical restriction pattern, while 6 fliI and 1 ureC specific amplicon produced different restriction pattern.
Furthermore, variations in fliI gene sequences in H.
pylori after treatment were also confirmed by sequencing and compared in silico.
Nested PCR based detection of H.
pylori is more sensitive method to detect H.
pylori after therapy than culture, RUT, and histology.
Further, this study suggests that H.
pylori is not eradicated completely after triple therapy.
American Psychological Association (APA)
Patel, Saurabh Kumar& Mishra, Girish Narayan& Pratap, Chandra Bhan& Jain, Ashok Kumar& Nath, Gopal. 2014. Helicobacter pylori Is Not Eradicated after Triple Therapy : A Nested PCR Based Study. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-475174
Modern Language Association (MLA)
Patel, Saurabh Kumar…[et al.]. Helicobacter pylori Is Not Eradicated after Triple Therapy : A Nested PCR Based Study. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-475174
American Medical Association (AMA)
Patel, Saurabh Kumar& Mishra, Girish Narayan& Pratap, Chandra Bhan& Jain, Ashok Kumar& Nath, Gopal. Helicobacter pylori Is Not Eradicated after Triple Therapy : A Nested PCR Based Study. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-475174
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475174