Inhibited Production of iNOS by Murine J774 Macrophages Occurs via a phoP-Regulated Differential Expression of NFκB and AP-1
Joint Authors
Foster, Neil
Hulme, Scott D.
Barrow, Paul A.
Source
Interdisciplinary Perspectives on Infectious Diseases
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-17
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Background.
There are no reported data to explain how Salmonella suppress nitrite ion production in macrophages or whether this phenomenon is unique to typhoidal or non-typhoidal serovars.
The aims of this study were, therefore, to investigate these phenomena.
Methods.
We measured survival of S.
typhimurium 14028 and its phoP mutant in murine J774 macrophages, cultured with or without interferon gamma.
We compared expression of inducible nitric oxide synthase (iNOS) mRNA and protein, and nitrite ion production and also examined binding of nuclear factor κB (NFκB) and activator protein 1 (AP-1) to macrophage DNA.
Results.
S.
typhimurium 14028 inhibited binding of NFκB and AP-1 to DNA in murine J774.
A macrophages via an intact phoP regulon.
This correlated with increased survival and reduced iNOS expression.
Suppression of NFκB activity was ameliorated in macrophages cultured with IFN-γ and this correlated with increased expression of iNOS mRNA and nitrite ion production, although IFN-γ had no effect on AP-1/DNA interaction.
We show, that with one exception, suppression of iNOS is unique to typhoidal serovars.
Conclusion.
S.
typhimurium inhibit NFκB and AP-1 interaction with macrophage DNA via the PhoP regulon, this reduces nitrite ion production and is principally associated with typhoidal serovars.
American Psychological Association (APA)
Hulme, Scott D.& Barrow, Paul A.& Foster, Neil. 2012. Inhibited Production of iNOS by Murine J774 Macrophages Occurs via a phoP-Regulated Differential Expression of NFκB and AP-1. Interdisciplinary Perspectives on Infectious Diseases،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-475178
Modern Language Association (MLA)
Hulme, Scott D.…[et al.]. Inhibited Production of iNOS by Murine J774 Macrophages Occurs via a phoP-Regulated Differential Expression of NFκB and AP-1. Interdisciplinary Perspectives on Infectious Diseases No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-475178
American Medical Association (AMA)
Hulme, Scott D.& Barrow, Paul A.& Foster, Neil. Inhibited Production of iNOS by Murine J774 Macrophages Occurs via a phoP-Regulated Differential Expression of NFκB and AP-1. Interdisciplinary Perspectives on Infectious Diseases. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-475178
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475178