Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats

Abstract EN

Organ/tissue transplantation has become an effective therapy for end-stage diseases.

However, immunosuppression after transplantation may cause severe side effects.

Donor-specific transplant tolerance was proposed to solve this problem.

In this study, we report a novel method for inducing and maintaining heart allograft tolerance rats.

First, we induced indefinite vascularized hind-limb allograft survival with a short-term antilymphocyte serum + Cyclosporine A treatment.

Peripheral blood chimerism disappeared 6-7 weeks after immunosuppression was withdrawn.

Then the recipients accepted secondary donor-strain skin and heart transplantation 200 days following vascularized hind-limb transplantation without any immunosuppression, but rejected third party skin allografts, a status of donor-specific tolerance.

The ELISPOT results suggested a mechanism of clone deletion.

These findings open new perspectives for the role of vascularized hind-limb transplant in the induction and maintenance of organ transplantation tolerance.