In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae
Joint Authors
Yang, Hai-Wei
Wei, Ji-Fu
Li, Xiaohong
Wu, Jing
Chen, Hao
Liu, Yehai
Source
International Journal of Genomics
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The house dust mites are major sources of indoor allergens for humans, which induce asthma, rhinitis, dermatitis, and other allergic diseases.
Der f 25 is a triosephosphate isomerase, representing the major allergen identified in Dermatophagoides farinae.
The objective of this study was to predict the B and T cell epitopes of Der f 25.
In the present study, we analyzed the physiochemical properties, function motifs and domains, and structural-based detailed features of Der f 25 and predicted the B cell linear epitopes of Der f 25 by DNAStar protean system, BPAP, and BepiPred 1.0 server and the T cell epitopes by NetMHCIIpan-3.0 and NetMHCII-2.2.
As a result, the sequence and structure analysis identified that Der f 25 belongs to the triosephosphate isomerase family and exhibited a triosephosphate isomerase pattern (PS001371).
Eight B cell epitopes (11–18, 30–35, 71–77, 99–107, 132–138, 173–187, 193–197, and 211–224) and five T cell epitopes including 26–34, 38–54, 66–74, 142–151, and 239–247 were predicted in this study.
These results can be used to benefit allergen immunotherapies and reduce the frequency of mite allergic reactions.
American Psychological Association (APA)
Li, Xiaohong& Yang, Hai-Wei& Chen, Hao& Wu, Jing& Liu, Yehai& Wei, Ji-Fu. 2014. In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae. International Journal of Genomics،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-475249
Modern Language Association (MLA)
Li, Xiaohong…[et al.]. In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae. International Journal of Genomics No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-475249
American Medical Association (AMA)
Li, Xiaohong& Yang, Hai-Wei& Chen, Hao& Wu, Jing& Liu, Yehai& Wei, Ji-Fu. In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae. International Journal of Genomics. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-475249
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475249