Activated Charcoal—A Potential Material in Glucoamylase Recovery
Joint Authors
Popoola, T. O. S.
Akpan, I.
Kareem, S. O.
Sanni, L. O.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-14
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
The potential of activated charcoal in the purification of fungal glucoamylase was investigated.
Various concentrations of activated charcoal (1–4% w/v) were used to concentrate crude glucoamylase from Rhizopus oligosporus at different temperature values (30–50°C).
Effects of pH (3.0–6.0) and contact time (0–60 min) on enzyme purification were also monitored.
Activated charcoal (3% w/v) gave a 16-fold purification in a single-step purification at 50°C for 20 min and pH 5.5.
The result of SDS-PAGE analysis of purified glucoamylase showed two major protein bands with corresponding molecular weight of 36 kDa and 50 kDa.
The method is inexpensive, rapid, and simple which could facilitate downstream processing of industrial enzyme.
American Psychological Association (APA)
Kareem, S. O.& Akpan, I.& Popoola, T. O. S.& Sanni, L. O.. 2011. Activated Charcoal—A Potential Material in Glucoamylase Recovery. Enzyme Research،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-475252
Modern Language Association (MLA)
Kareem, S. O.…[et al.]. Activated Charcoal—A Potential Material in Glucoamylase Recovery. Enzyme Research No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-475252
American Medical Association (AMA)
Kareem, S. O.& Akpan, I.& Popoola, T. O. S.& Sanni, L. O.. Activated Charcoal—A Potential Material in Glucoamylase Recovery. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-475252
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475252