Activated Charcoal—A Potential Material in Glucoamylase Recovery

Joint Authors

Popoola, T. O. S.
Akpan, I.
Kareem, S. O.
Sanni, L. O.

Source

Enzyme Research

Issue

Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-12-14

Country of Publication

Egypt

No. of Pages

4

Main Subjects

Biology

Abstract EN

The potential of activated charcoal in the purification of fungal glucoamylase was investigated.

Various concentrations of activated charcoal (1–4% w/v) were used to concentrate crude glucoamylase from Rhizopus oligosporus at different temperature values (30–50°C).

Effects of pH (3.0–6.0) and contact time (0–60 min) on enzyme purification were also monitored.

Activated charcoal (3% w/v) gave a 16-fold purification in a single-step purification at 50°C for 20 min and pH 5.5.

The result of SDS-PAGE analysis of purified glucoamylase showed two major protein bands with corresponding molecular weight of 36 kDa and 50 kDa.

The method is inexpensive, rapid, and simple which could facilitate downstream processing of industrial enzyme.

American Psychological Association (APA)

Kareem, S. O.& Akpan, I.& Popoola, T. O. S.& Sanni, L. O.. 2011. Activated Charcoal—A Potential Material in Glucoamylase Recovery. Enzyme Research،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-475252

Modern Language Association (MLA)

Kareem, S. O.…[et al.]. Activated Charcoal—A Potential Material in Glucoamylase Recovery. Enzyme Research No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-475252

American Medical Association (AMA)

Kareem, S. O.& Akpan, I.& Popoola, T. O. S.& Sanni, L. O.. Activated Charcoal—A Potential Material in Glucoamylase Recovery. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-475252

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-475252