Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing
Joint Authors
Sung, Jung-Suk
Cho, Sungbo
Koh, Hyun Sook
Kim, Donghee
Park, Hyo Eun
Kim, Jae Young
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Stem cells are useful for cell replacement therapy.
Stem cell differentiation must be monitored thoroughly and precisely prior to transplantation.
In this study we evaluated the usefulness of electric cell-substrate impedance sensing (ECIS) for in vitro real-time monitoring of neural differentiation of human mesenchymal stem cells (hMSCs).
We cultured hMSCs in neural differentiation media (NDM) for 6 days and examined the time-course of impedance changes with an ECIS array.
We also monitored the expression of markers for neural differentiation, total cell count, and cell cycle profiles.
Cellular expression of neuron and oligodendrocyte markers increased.
The resistance value of cells cultured in NDM was automatically measured in real-time and found to increase much more slowly over time compared to cells cultured in non-differentiation media.
The relatively slow resistance changes observed in differentiating MSCs were determined to be due to their lower growth capacity achieved by induction of cell cycle arrest in G0/G1.
Overall results suggest that the relatively slow change in resistance values measured by ECIS method can be used as a parameter for slowly growing neural-differentiating cells.
However, to enhance the competence of ECIS for in vitro real-time monitoring of neural differentiation of MSCs, more elaborate studies are needed.
American Psychological Association (APA)
Park, Hyo Eun& Kim, Donghee& Koh, Hyun Sook& Cho, Sungbo& Sung, Jung-Suk& Kim, Jae Young. 2011. Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-475355
Modern Language Association (MLA)
Park, Hyo Eun…[et al.]. Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-475355
American Medical Association (AMA)
Park, Hyo Eun& Kim, Donghee& Koh, Hyun Sook& Cho, Sungbo& Sung, Jung-Suk& Kim, Jae Young. Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-475355
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475355