The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT
Joint Authors
Lii, Chong-Kuei
Tsai, Jen-Hsiang
Chen, Yi-Wan
Liu, Jer-Yuh
Chen, Haw-Wen
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We investigated the effect of vitamin E on membrane protein thiols under oxidative stress, which we induced by treating hepatocytes with tert-butyl hydroperoxide (TBH) for 60 mins.
Those cells which we pretreated with vitamin E formed fewer blebs (22.3% compared to 60.0% in nonvitamin E-treated cells) and maintained cytosolic calcium concentration and the number of membrane protein thiols instead of showing the usual symptoms in cells undergoing oxidative stress.
Dithiothreitol (DTT) also commonly reduces bleb formation in hepatocytes affected by TBH.
However, our experiments clearly demonstrate that DTT does not prevent the changes in cytosolic calcium and membrane protein thiols in the blebbing cells.
Consequently, we decided to pretreat cells with both DTT and vitamin E and found that the influence of TBH was entirely prevented.
These findings may provide us with a new aspect for investigating the mechanism of bleb formation under oxidative stress.
American Psychological Association (APA)
Tsai, Jen-Hsiang& Chen, Haw-Wen& Chen, Yi-Wan& Liu, Jer-Yuh& Lii, Chong-Kuei. 2010. The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-475470
Modern Language Association (MLA)
Tsai, Jen-Hsiang…[et al.]. The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-475470
American Medical Association (AMA)
Tsai, Jen-Hsiang& Chen, Haw-Wen& Chen, Yi-Wan& Liu, Jer-Yuh& Lii, Chong-Kuei. The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-475470
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475470