Antigenotoxic and Apoptotic Activity of Green Tea Polyphenol Extracts on Hexavalent Chromium-Induced DNA Damage in Peripheral Blood of CD-1 Mice : Analysis with Differential Acridine OrangeEthidium Bromide Staining
Joint Authors
Carvente-Juárez, Megumi Monserrat
Altamirano-Lozano, Mario Agustín
García-Rodríguez, María del Carmen
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
This study was conducted to investigate the modulating effects of green tea polyphenols on genotoxic damage and apoptotic activity induced by hexavalent chromium [Cr (VI)] in CD-1 mice.
Animals were divided into the following groups: (i) injected with vehicle; (ii) treated with green tea polyphenols (30 mg/kg) via gavage; (iii) injected with CrO3 (20 mg/kg) intraperitoneally; (iv) treated with green tea polyphenols in addition to CrO3.
Genotoxic damage was evaluated by examining micronucleated polychromatic erythrocytes (MN-PCEs) obtained from peripheral blood at 0, 24, 48, and 72 h after treatment.
Induction of apoptosis and cell viability were assessed by differential acridine orange/ethidium bromide (AO/EB) staining.
Treatment of green tea polyphenols led to no significant changes in the MN-PCEs.
However, CrO3 treatment significantly increased MN-PCEs at 24 and 48 h after injection.
Green tea polyphenols treatment prior to CrO3 injection led to a decrease in MN-PCEs compared to the group treated with CrO3 only.
The average of apoptotic cells was increased at 48 h after treatment compared to control mice, suggesting that apoptosis could contribute to eliminate the DNA damaged cells induced by Cr (VI).
Our findings support the proposed protective effects of green tea polyphenols against the genotoxic damage induced by Cr (VI).
American Psychological Association (APA)
García-Rodríguez, María del Carmen& Carvente-Juárez, Megumi Monserrat& Altamirano-Lozano, Mario Agustín. 2013. Antigenotoxic and Apoptotic Activity of Green Tea Polyphenol Extracts on Hexavalent Chromium-Induced DNA Damage in Peripheral Blood of CD-1 Mice : Analysis with Differential Acridine OrangeEthidium Bromide Staining. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-475489
Modern Language Association (MLA)
García-Rodríguez, María del Carmen…[et al.]. Antigenotoxic and Apoptotic Activity of Green Tea Polyphenol Extracts on Hexavalent Chromium-Induced DNA Damage in Peripheral Blood of CD-1 Mice : Analysis with Differential Acridine OrangeEthidium Bromide Staining. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-475489
American Medical Association (AMA)
García-Rodríguez, María del Carmen& Carvente-Juárez, Megumi Monserrat& Altamirano-Lozano, Mario Agustín. Antigenotoxic and Apoptotic Activity of Green Tea Polyphenol Extracts on Hexavalent Chromium-Induced DNA Damage in Peripheral Blood of CD-1 Mice : Analysis with Differential Acridine OrangeEthidium Bromide Staining. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-475489
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475489