Isolation and Characterization of Adhesive Secretion from Cuvierian Tubules of Sea Cucumber Holothuria forskåli (Echinodermata : Holothuroidea)
Joint Authors
Schloßmacher, Ute
Schröder, Heinz C.
McKenzie, J. Douglas
Müller, Werner E. G.
Baranowska, Malgorzata
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-25
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The sea cucumber Holothuria forskåli possesses a specialized system called Cuvierian tubules.
During mechanical stimulation white filaments (tubules) are expelled and become sticky upon contact with any object.
We isolated a protein with adhesive properties from protein extracts of Cuvierian tubules from H.
forskåli.
This protein was identified by antibodies against recombinant precollagen D which is located in the byssal threads of the mussel Mytilus galloprovincialis.
To find out the optimal procedure for extraction and purification, the identified protein was isolated by several methods, including electroelution, binding to glass beads, immunoprecipitation, and gel filtration.
Antibodies raised against the isolated protein were used for localization of the adhesive protein in Cuvierian tubules.
Immunostaining and immunogold electron microscopical studies revealed the strongest immunoreactivity in the mesothelium; this tissue layer is involved in adhesion.
Adhesion of Cuvierian tubule extracts was measured on the surface of various materials.
The extracted protein showed the strongest adhesion to Teflon surface.
Increased adhesion was observed in the presence of potassium and EDTA, while cadmium caused a decrease in adhesion.
Addition of antibodies and trypsin abolished the adhesive properties of the extract.
American Psychological Association (APA)
Baranowska, Malgorzata& Schloßmacher, Ute& McKenzie, J. Douglas& Müller, Werner E. G.& Schröder, Heinz C.. 2011. Isolation and Characterization of Adhesive Secretion from Cuvierian Tubules of Sea Cucumber Holothuria forskåli (Echinodermata : Holothuroidea). Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-475518
Modern Language Association (MLA)
Baranowska, Malgorzata…[et al.]. Isolation and Characterization of Adhesive Secretion from Cuvierian Tubules of Sea Cucumber Holothuria forskåli (Echinodermata : Holothuroidea). Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-475518
American Medical Association (AMA)
Baranowska, Malgorzata& Schloßmacher, Ute& McKenzie, J. Douglas& Müller, Werner E. G.& Schröder, Heinz C.. Isolation and Characterization of Adhesive Secretion from Cuvierian Tubules of Sea Cucumber Holothuria forskåli (Echinodermata : Holothuroidea). Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-475518
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475518