Bacterial Cells Carrying Synthetic Dual-Function Operon Survived Starvation
Joint Authors
Matsumoto, Yuki
Ito, Yoichiro
Tsuru, Saburo
Yomo, Tetsuya
Ying, Bei-Wen
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A synthetic dual-function operon with a bistable structure was designed and successfully integrated into the bacterial genome.
Bistability was generated by the mutual inhibitory structure comprised of the promoters Ptet and Plac and the repressors LacI and TetR.
Dual function essential for cell growth was introduced by replacing the genes (i.e., hisC and leuB) encoding proteins involved in the biosynthesis of histidine and leucine from their native chromosomal locations to the synthetic operon.
Both colony formation and population dynamics of the cells carrying this operon showed that the cells survived starvation and the newly formed population transited between the two stable states, representing the induced hisC and leuB levels, in accordance with the nutritional status.
The results strongly suggested that the synthetic design of proto-operons sensitive to external perturbations is practical and functional in native cells.
American Psychological Association (APA)
Matsumoto, Yuki& Ito, Yoichiro& Tsuru, Saburo& Ying, Bei-Wen& Yomo, Tetsuya. 2011. Bacterial Cells Carrying Synthetic Dual-Function Operon Survived Starvation. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-475657
Modern Language Association (MLA)
Matsumoto, Yuki…[et al.]. Bacterial Cells Carrying Synthetic Dual-Function Operon Survived Starvation. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-475657
American Medical Association (AMA)
Matsumoto, Yuki& Ito, Yoichiro& Tsuru, Saburo& Ying, Bei-Wen& Yomo, Tetsuya. Bacterial Cells Carrying Synthetic Dual-Function Operon Survived Starvation. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-475657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475657