Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus
Joint Authors
Leivo, Hilary E.
Guillemette, J. Guy
Dupont, Andrea L.
Montgomery, Heather J.
Source
Biochemistry Research International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-30
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
The nitric oxide synthase-like protein from Bacillus cereus (bcNOS) has been cloned, expressed, and characterized.
This small hemeprotein (356 amino acids in length) has a mass of 43 kDa and forms a dimer.
The recombinant protein showed similar spectral shifts to the mammalian NOS proteins and could bind the substrates L-arginine and NG-hydroxy-L-arginine as well as the ligand imidazole.
Low levels of activity were recorded for the hydrogen peroxide-dependent oxidation of NG-hydroxy-L-arginine and L-arginine by bcNOS, while a reconstituted system with the rat neuronal NOS reductase domain showed no activity.
The recombinant bcNOS protein adds to the complement of bacterial NOS-like proteins that are used for the investigation of the mechanism and function of NO in microorganisms.
American Psychological Association (APA)
Montgomery, Heather J.& Dupont, Andrea L.& Leivo, Hilary E.& Guillemette, J. Guy. 2009. Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus. Biochemistry Research International،Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-475721
Modern Language Association (MLA)
Montgomery, Heather J.…[et al.]. Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus. Biochemistry Research International No. 2010 (2010), pp.1-4.
https://search.emarefa.net/detail/BIM-475721
American Medical Association (AMA)
Montgomery, Heather J.& Dupont, Andrea L.& Leivo, Hilary E.& Guillemette, J. Guy. Cloning, Expression, and Purification of a Nitric Oxide Synthase-Like Protein from Bacillus cereus. Biochemistry Research International. 2009. Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-475721
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-475721